Mechanisms of inhibited lipolytic response to isoproterenol. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antidiabetic effect of sida cordata in alloxan induced diabetic rats. Enhancement of bilirubin excretion in alloxan induced diabetic rats treated with crude aloe vera gel u. Since carbohydrate metabolism is modified in diabetic animals, its of interest to determine the effect of tryptophan on alloxan induced diabetic rat. Blood glucose level and lipid profile of alloxaninduced. In diabetic rats, the fasting blood glucose level, indexes of heart and liver, and levels of cholesterol and triglyceride in serum and mda in heart. The results revealed a significant elevation in thiobarbituric acid reactive substances tbars levels in the brain and testes of diabetic rats. Antioxidant effect of curcumin extracts in induced diabetic.
The mechanisms of alloxan and streptozotocininduced. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Osim2 1department of physiology, faculty of basic medical sciences, college of health sciences, university of uyo, uyo, akwa ibom state. Morin treatment attenuated hyperalgesia and analgesia p diabetic rats at both doses significantly decreased the levels of cytokines p diabetic neuropathy induced by injection with streptozotocin stz. Periodontal disease in diabetic patients presents higher severity and prevalence. We thus tested in this work whether a prolonged treatment with tyramine could improve glucose tolerance in streptozotocininduced diabetic rats.
Experimental model of induction of diabetes mellitus in rats. Diabetes was induced in the second, third and fourth groups by. It has been successfully induced in a variety of animal species. Early renal histological changes in alloxaninduced diabetic rats mohsen pourghasem 1, ebrahim nasiri 2, hamid shafi 3 1. Morin treatment attenuated hyperalgesia and analgesia p topiramate, levetiracetam and zonisamide in vincristine induced neuropathic pain in albino rats using chemical method punam jakhar1, omi chouhan2, anusuya gehlot 2, r. Streptozotocin induced experimental diabetes male wistar rats pdf. Research article alloxan induced diabetes mellitus in. It is better to standardize it first by jain et al. Adult male rats 8 weeks, weighing 195 to 225 g was made diabetic by injecting stz 65 mg kg1 body weight intraperitoneally. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Evaluation of antidiabetic activity of ginkgo biloba in streptozotocin. Antidiabetic activity of date seed methanolic extracts in alloxan induced diabetic rats.
Streptozotocin induced diabetes in wistar albino rats is neither type i nor type ii. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxaninduced diabetic rats. Ameliorative potential of morin in streptozotocininduced. Variables in alloxaninduced and fructose induced diabetic rats adesoji a fasanmade and oluwakemi t alabi departments of physiology and medicine college of medicine, university of ibadan ibadan, nigeria abstract honey contains a high concentration of fructose, a monosaccharide, capable of raising blood sugar level after oral ingestion. Antidiabetic activity of mediumpolar extract from the. A crude extract of carica papaya seeds was prepared in boiling water and the aqueous extract was dried.
This study aimed to determine the protective effect of curcumin on streptozotocin stz induced oxidative stress in various tissues of albino wister rats. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxan induced diabetic rats. Variables in alloxan induced and fructose induced diabetic rats adesoji a fasanmade and oluwakemi t alabi departments of physiology and medicine college of medicine, university of ibadan ibadan, nigeria abstract honey contains a high concentration of fructose, a monosaccharide, capable of raising blood sugar level after oral ingestion. Rathore1, raghuveer choudhary2, rajesh chaudhary3 1. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. The 4th and 5th diabetic groups were given glibenclamide 5. Effect of prolonged treatmen with tyramine on glucose. Forty male rats were uninephrectomized from the left flank. Intravenous injection of alloxan into rabbits, rats, dogs, monkeys, etc. Early renal histological changes in alloxan induced diabetic rats mohsen pourghasem 1, ebrahim nasiri 2, hamid shafi 3 1. A comparative study of the effect of gabapentin, topiramate. Jun 25, 2009 stz and alloxan induced diabetic mice hi im working in animal models mice. Alloxaninduced diabetes triggers the development of. In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and.
Early renal histological changes in alloxaninduced. Alloxan induced diabetic rats were divided into 5 groups of 5 each and were treated with 100, 200 and 400 mgkg of the methanol extract. To model diabetes type 2 a low dose of stz may be used in week old rats. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxan induced diabetic male rats. Antihyperglycaemic activity of cassia kleinii leaf extract in glucose fed normal rats and alloxan induced diabetic rats v babu, t gangadevi, a subramoniam. Experimental models play an important role in understanding such a disease, which is treatable only. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. This study describes a rat diabetes mellitus model induced by administering a reduced dose of alloxan, thus greatly reducing the animals death rate. This study was conducted to investigate the effect of nicotinamide on experimentally induced iddm. Effect of bauhinia holophylla treatment in streptozotocininduced. Anti diabetic activity of ethanolic extract of aconitum ferox in alloxan induced diaseased rats j kondal reddy1, p sheetal2, rajesh menda2, nukala aparna3 1department of pharmacology, vision college of pharmaceutical sciences, boduppal, hyderabad, india. Which type of diabetes will be induced by streptozotocin in.
The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. Alloxan induced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16. Antidiabetic effect of morinda lucida stem bark extracts on alloxaninduced diabetic rats. Healthy rats were confirmed as diabetic after treatment with alloxan. Data show that treatment with the extract drastically reduced the glucose level. A streptozotocin stz induced diabetes in animal model mimics, in some aspects, recent onset iddm. These findings suggest that morphologic aberrations are provoked by untreated diabetes mellitus, even in the absence of oxidative stress. Biochemical study on the hypoglycemic effects of onion and. Department of anatomical sciences, gilan university of medical sciences, rasht, iran. Effect of nicotinamide on experimental induced diabetes. The mechanism of alloxan and streptozotocin action in b cells. Anti diabetic activity of ethanolic extract of aconitum ferox. Mechanisms of inhibited lipolytic response to isoproterenol by metformin in primary rat adipocytes this study is to investigate this study is to investigate the inhibitory effects of metformin on isoproterenol induced lipolysis in primary rat adipocytes and further elucidate the.
This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Rosmarinic acid ameliorates diabetic nephropathy in. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets.
Autoxidation of dialuric acid generates superoxide radicals. Stz and alloxan induced diabetic mice scientist solutions. We read with interest the article titled anomalies in alloxaninduced diabetic model. Early renal histological changes in alloxaninduced diabetic. A streptozotocin stzinduced diabetes in animal model mimics, in some aspects, recent onset iddm. In alloxan induced diabetic rats by oral administration of extract 100mgkg body weight. What is the best method to induce type 2 diabetes rat model. The use of experimentally induced diabetes in rats remains as one of the easiest and convenient methods for screening of new drugs. The protective effect of nigella sativa against cisplatin. The effects of ethanol extracts on glucose tolerance tests were significant.
The diabetic state induced by streptozotocin injection is known to impair oligodendroglial. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. First, tyramine content of standard rodent chow was determined by hplc and daily tyramine intake of control rats was estimated to. It is used as a cleansing and purifying agent and yet can be considered as a bodybuilder because of its nutritional value. This was closely associated with a concomitant reduction in levels of stt and reduced testes weight, a noticeable decline in the glutathionestransferase gst. Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. Antidiabetic activity of vinca rosea extracts in alloxan. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
Diabetes induction and animals selection were followed as described in glucose tolerance test in diabetic. Single dose study after single dose of the mesc 200 or 400 mgkg, p. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. At a dose of 100 mgkg, 200mgkg the extract was given to male sprague dawley rats for 14 days to evaluate the anti hyperglycemic and anti hyperlipidaemic activity in streptozotocin. Hence it is vital to establish a dosage of drugs used in induction of experimental diabetes so as to develop stable diabetic characteristics with minimal drug toxicity. This study was conducted to investigate the effect of nicotinamide on experimentallyinduced iddm. The protective effect of nigella sativa against cisplatininduced nephrotoxicity in rats sara hosseinian1, abolfazl 2khajavi rad, mousaalreza hadjzadeh3, nema mohamadian roshan4, shahrzad havakhah1, somayeh shafiee1 1department of physiology, school of medicine, mashhad university of medical sciences, mashhad, iran.
Adeniyi paulina oludoyin 1, 2, sanusi rasaki adegoke 1. Alloxaninduced diabetes, a common model for evaluating. Wheatgrass has many characteristics desirable for health. Antidiabetic effect of morinda lucida stem bark extracts on. The mechanisms of alloxan and streptozotocininduced diabetes. Thirty spraque dawley rats were divided into 3 groups. Treatment of alloxaninduced diabetic rats with metformin or. Bioactive extracts of moringa reverted glucose level to normal in alloxan induced diabetic rats.
Neurotoxicity induced by a single oral dose of aniline in rats. The diabetic rats were treated with moringa extract as described in section 2. Functional consequences after buserelininduced enteric. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxaninduced diabetic male rats. This study aimed to determine the protective effect of curcumin on streptozotocin stzinduced oxidative stress in various tissues of albino wister rats. This study is aimed to evaluate the antidiabetic activity of ethanolic extracts of s. A closed fracture was performed on the left tibia of 3monthold wistar rats weighing 250 to 350 g that were either healthy n 24 or made diabetic with alloxan. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Early renal histological changes in alloxaninduced diabetic rats. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. Nitric oxide and inflammatory cytokine productions in diabetic rats supplemented with goat milk and soy milk kefir. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic.
Antihyperglycaemic activity of cassia kleinii leaf extract. Palliative effects of daonil supplemented with tryptophan on. Anti diabetic effect of cherries in alloxan induced diabetic rats. Enhancement of bilirubin excretion in alloxan induced. Cellular and molecular biology research center cmbrc, babol university of medical sciences, babol, iran. Subjects a total of 254 male spraguedawley rats 250300g, obtained from. Bertoni on alloxaninduced dia betic rats bertoni on alloxaninduced dia betic rats himanshu misra, 1, 2 manish soni, 3 narendra silawat, 4 darshana mehta, 1 b. Nitric oxide and inflammatory cytokine productions in. Original article modulatory influence of parkia biglobosa.
The endpoint measures are blood glucose levels, body weight, and a standard behavioral measure of pain threshold. Comparison of streptozotocin and alloxaninduced diabetes in. Alloxaninduced diabetes delays repair in a rat model of. Hypoglycemic effects in alloxaninduced diabetic rats of the. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on. Histopathological and immunohistochemical study of antidiabetic. Fauna 3 kampus ugm, bulaksumur, yogyakarta 55281, indonesia. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. At a dose of 100 mgkg, 200mgkg the extract was given to male sprague dawley rats for 14 days to evaluate the anti hyperglycemic and anti hyperlipidaemic activity in streptozotocin nicotinamide induced diabetic rats. In the majority of experiments, na is given to rats 15 min before stz to induce niddm. Bioactive extracts of moringa reverted glucose level to normal in alloxaninduced diabetic rats. Differential effect of honey on selected variables in alloxan.
A comparative study of the effect of gabapentin, topiramate, levetiracetam and zonisamide for neuropathic pain induced by anticancer drug vincristine in rats punam jakhar1, omi chouhan1, anusuya gehlot1, r. The rs504393 influences the level of nociceptive factors. Anion gap toxicity in alloxan induced type 2 diabetic rats. Effect of ginger zingiber officinale extracts on blood glucose in normal and streptozotocininduced diabetic rats. Pdf phytochemicals, hypoglycemic and hypolipidemic.
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